The Map Manager time-series panel provides an interface to load, save and browse Map Manager time-series (maps). Open the time-series panel using the menu ‘MapManager - Time Series’
Loaded maps are listed on the left and when a map is selected, its time-points (stacks) are listed on the right.
- Open Map. Open/Load a map from the hard-drive.
- Save Map. Save selected map to hard-drive. By default, only the portions of the map that need saving are saved. Ctrl+click will save the entire map.
Opening Maps. When opening a map, select the maps '.ipf' file. If a map is named 'mymap', load 'mymap.ipf'.
- Object Map. Open a map plot. For spines, this will plot the position of each spine along its dendritic segment versus time-points (Sessions). For other annotations, this will plot annotations in their creation order. In both cases, these plots are crucial tools to visualize annotation dynamics.
- Segment Map. Open a map plot showing the segments within each session and the connectivity of segments across sessions. This is only for spine annotations that have segment tracings.
- X Axis. Choose different X-Axis for Object and Segment map plots. Available X-Axis are:
- Zero Sessions
- Zero Days
- Zero Hours
- X-Axis Condition.
Datetime, Days and Hours require stacks to have a date/time specified. A stacks date/time can be manually edited by turning on ‘Edit Date/Time’ in the Utility Tab. The X-Axis options ‘Zero Sessions’, ‘Zero Days’, ‘Zeros Hours’ use a session in the map as a zero timepoint. Specify this by right-clicking on a stack and selecting ‘Zero Session’. The zero session for a map will have a ‘*’ in its session list. Zero session is also used when plotting from time-series panel tabs including: Dynamics, Trajectory, and Intensity.
- Search. Open the search panel.
- Plot. Open the plot panel.
- Pool. Open the map pool panel.
- Map DB. Open a map database panel to quickly browse and load maps from hard-drive. No documentation yet.
- Unload Stacks. Unload all stacks in the selected map. Ctrl+click to unload all stacks in all open maps. Unloading a stack will unload the RAW IMAGE DATA. It DOES NOT unload the stack annotations or the map.
- Close Windows. Close all windows associated with the selected map.
Right-click for context menus
Right-click on a map name.
- Find Points. Open find points panel.
- Density Report. Generate a density report for the map. See reports.
- Dynamics Report. Generate a dynamics report for the map. See reports.
- Survival Report. Generate a survival report for the map. See reports.
- Export Map. DO NOT USE Exports a map to text files. Files are saved in the ‘export/’ folder.
- Show on HDD. Shows the hard-drive folder where the map is saved.
- Close map. Close a map, removing all associated images and analysis from Igor memory.
- Close Windows. Close all open windows associated with a map.
Right-click on a stack name.
- Display Stack. Display the stack in a stack window.
- Plot Run +-1. Plot a run of stacks from the selected stack.
- Plot Run +- All.
- Plot Run +- n.
- Zero Session. Set the selected stack to the zero session. Zero sessions are used when plotting a map with X-Axis set to ‘Zero Session’, ‘Zero Days’, and ‘Zero Hours’. The zero session will have an Asterix (*) before its session index in the list of sessions.
- Find Points. Open find points panel.
- Edit Table. Edit annotations in a stack as a text table. This is useful to copy and paste annotations for further analysis.
- Unload Stack. Unload the raw image data for a stack. Unloading a stack does not unload the stack annotations.
Right-click on the headers in the stack list.
- Columns can be toggled on and off.
- Edit Condition. Turns on editing of map and session conditions.
Interface to create maps and manage time-points. See making a map.
Interface to plot the dynamics of annotations. See reports to create tabular reports of annotation dynamics.
Interface to analyze and plot the trajectories of annotations.
Interface to plot intensity analysis of spine annotations. See intensity.
Right-click on plot buttons to plot ‘lifetime’ and ‘death’.
Interface for advanced and internal debugging. In general there is no need to use this.