- Mouse wheel to scroll up and down through images, arrow keys to pan, +/- to zoom
- Image contrast is critical, open contrast bar with keyboard ‘c’, adjust min/max contrast with sliders.
- View a histogram with keyboard ‘h’
- Closing a stack window does not remove it from memory (the stack remains open)
Arrow keys : pan image left/right and up/down + : zoom in (follows mouse pointer) - : zoom out (follows mouse pointer) Return/Enter : view entire image Esc : cancel selection 1 : view channel 1 2 : view channel 2 3 : view a sliding maximal z-projection of channel 1 4 : view a sliding maximal z-projection of channel 2 c : toggle contrast control bar h : open histogram window ] : toggle between two different window sizes sift+c : cycle through 4 different window 'candy' displays shift+p : Set pixel/voxel size shift+? : open the 'Point Info' window Object notes can be set in the Point Info window.
- Open the contrast bar with keyboard ‘c’
- Pixel intensity values below ‘min’ will be displayed as black
- Pixel intensity values above ‘max’ will be displayed as white
The histogram window show a histogram of pixel intensity values for each image in a stack.
- Open the histogram window with keyboard ‘h’.
- The histogram window shows a pixel intensity histogram for one image.
- Scroll through the image in a stack (mouse-wheel) and the histogram for each image will be show.
The navigation window opens a new window with a maximal z-projection of the stack and shows the current zoomed view as a blue square.
- Right click and select ‘Navigation Window’ menu. A navigation window will be opened and your current zoomed view of the stack will be shown as a blue square.
- Zoom with +/- or pan with arrow keys and the blue-square tracks what you are looking at.
- The navigation window is, by default, a maximal z-projection of the entire stack.
The Point Info window shows information about the currently selected object.
- Open the Point Info window from any stack window using shift+?.
- Use the point info window to annotate either nodes or edges with notes.
- Use the point info window to annotate edges as ‘diving’, ‘surface’, ‘Has Pericyte’ and ‘Branch Order’.
- Contrast is critical, if not set properly you will not see objects in your image that are actually there.
- Closing a stack window does not remove the stack from memory.
- Zooming with keyboard +/- follows the mouse pointer